The team then remove one pronucleus and attempt to fertilise it by injecting sperm.
From the newscientist.com
The pronucleus microinjection method is the most commonly used.
From the sciencedaily.com
The spermatozoon which fertilizes an oocyte will contribute its pronucleus, the other half of the zygotic genome.
From the en.wikipedia.org
When the egg is fertilised, it keeps half of its chromosomes in its so-called pronucleus, and spits out the rest in a little package called the polar body.
From the newscientist.com
The standard way to insert genes into animals is to physically inject the genes through a fine needle into the pronucleus of a newly fertilised egg.
From the newscientist.com
The first method is somewhat easier because the nuclear DNA of a fertilized egg is neatly contained in a pronucleus, while the DNA of an unfertilized egg isn't.
From the businessweek.com
More examples
The nucleus of the ovum or sperm after fertilization but before they fuse to form the nucleus of the zygote
(Pronuclei) The nuclear material found in the egg (female pronucleus) or enclosed in the head of the sperm (male pronucleus). Two pronuclei in the egg cytoplasm indicate that fertilization has occurred.
(pronuclei) Two haploid nuclei in a newly fertilized egg.
Haploid, gametic nucleus (from sperm or egg) in a fertilized egg, prior to fusion to form a zygote nucleus
Either of the 2 haploid gamete nuclei just prior to their fusion in the fertilized ovum. Transgenic lines are often generated by microinjection of the transgene into the pronuclear region of these haploid gametes.
The male and female nuclei that are visible 14-18 hours after combining sperm with an egg